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Use of PCR-DGGE based molecular methods to analyse microbial community diversity and stability during the thermophilic stages of an ATAD wastewater sludge treatment process as an aid to performance monitoring.

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dc.contributor.author Piterina, Anna Valentinova
dc.contributor.author Pembroke, Tony J.
dc.date.accessioned 2016-04-13T14:46:31Z
dc.date.available 2016-04-13T14:46:31Z
dc.date.issued 2013
dc.identifier.uri http://hdl.handle.net/10344/5015
dc.description peer-reviewed en_US
dc.description.abstract PCR and PCR-DGGE techniques have been evaluated to monitor biodiversity indexes within an ATAD (autothermal thermophilic aerobic digestion) system treating domestic sludge for land spread, by examining microbial dynamics in response to elevated temperatures during treatment. The ATAD process utilises a thermophilic population to generate heat and operates at elevated pH due to degradation of sludge solids, thus allowing pasteurisation and stabilisation of the sludge. Genera-specific PCR revealed that Archaea, Eukarya and Fungi decline when the temperature reaches 59∘C, while the bacterial lineage constitutes the dominant group at this stage. The bacterial community at the thermophilic stage, its similarity index to the feed material, and the species richness present were evaluated by PCR-DGGE. Parameters such as choice of molecular target (16S rDNA or rpoB genes), and electrophoresis condition, were optimised to maximise the resolution of the method for ATAD. Dynamic analysis of microbial communities was best observed utilising PCR-DGGE analysis of the V6-V8 region of 16S rDNA, while rpoB gene profiles were less informative. Unique thermophilic communities were shown to quickly adapt to process changes, and shown to be quite stable during the process. Such techniques may be used as a monitoring technique for process health and efficiency. en_US
dc.language.iso eng en_US
dc.publisher Hindawi Publishing Corporation en_US
dc.relation.ispartofseries BMC Biotechnology;article ID 162645
dc.relation.uri http://pembrokelab.wordpress.com/current-works/
dc.subject PCR DGGE ATAD en_US
dc.subject thermophilic community diversity en_US
dc.title Use of PCR-DGGE based molecular methods to analyse microbial community diversity and stability during the thermophilic stages of an ATAD wastewater sludge treatment process as an aid to performance monitoring. en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.date.updated 2016-04-13T14:37:59Z
dc.description.version PUBLISHED
dc.contributor.sponsor HEA en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 1444741
dc.internal.copyrightchecked Yes
dc.description.status peer-reviewed


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