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Increased intestinal permeability in rats subjected to traumatic frontal lobe percussion brain injury

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Show simple item record Feighery, Linda Smith, Aoife Keely, Simon Baird, Alan W. O'Connor, William T. Callanan, John J. Brayden, David J. 2015-09-22T16:08:26Z 2015-09-22T16:08:26Z 2008
dc.description peer-reviewed en_US
dc.description.abstract Background: Dysfunction of the gastrointestinal-(Gl} tracns-a common-occurrence following traumatic brain injury (TBI). We hypothesised that increased intestinal permeability may result from a precisely controlled percussion injury to the exposed brains of anaesthetised rats and that such an effect could be assessed in vitro using excised intestinal mucosae mounted in Ussing chambers. Methods: Following craniotomy over the left medial prefrontal cortex on anaesthetised rats, neurotrauma was produced using a pneumatically-driven impactor on the exposed brain. Control rats were subjected to identical procedures but did not receive an impact. Muscle-stripped rat intestinal ileal and colonic segments were mounted in Ussing chambers within 30 minutes of death. Transepithelial electrical resistance (TEER) and the apparent permeability coefficient (Papp) of[14C)-mannitol were recorded from intestinal tissue for 120 minutes. Histopathology was also carried out to determine any gross morphological changes in the intestine. Results: Ileal and colonic mucosae showed no differences in TEER in ileum or colon ofTBI rats compared to controls. The Papp of mannitol was significantly increased in ilea from rats previously exposed to TBI compared to controls. Histological analysis showed gross changes to 50% of the ileal but not the colonic sections from TBI rats. Conclusion: TBI results in significantly reduced ileal barrier function, most likely mediated by open tight junctions. For patients with acute head injury, this may have implications for subsequent oral absorption of nutrients. Systemic delivery of luminal endotoxins may contribute to multiple organ failure. en_US
dc.language.iso eng en_US
dc.publisher Lippincott, Williams and Wilkins en_US
dc.relation.ispartofseries Journal of Trauma-Injury Infection and Critical Care;64, (1), pp. 131-138
dc.rights This is an Author's Original Manuscript of an article whose final and definitive form, the Version of Record, has been published in the Journal of Trauma-Injury Infection and Critical Care, 2008 64 (1), pp. 131-138, . © Lippincott, Williams and Wilkins en_US
dc.subject traumatic brain injury en_US
dc.subject intestinal permeability en_US
dc.subject gut-brain axis en_US
dc.title Increased intestinal permeability in rats subjected to traumatic frontal lobe percussion brain injury en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.contributor.sponsor HRB en_US
dc.contributor.sponsor SFI en_US
dc.contributor.sponsor National Neuroscience Network (Ireland) en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US

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