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Promoting cell proliferation using water dispersible germanium nanowires.

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dc.contributor.author Bezuidenhout, Michael
dc.contributor.author Liu, Pai
dc.contributor.author Singh, Shalini
dc.contributor.author Kiely, Maeve
dc.contributor.author Ryan, Kevin M.
dc.contributor.author Kiely, Patrick A.
dc.date.accessioned 2014-11-24T19:01:11Z
dc.date.available 2014-11-24T19:01:11Z
dc.date.issued 2014
dc.identifier.uri http://hdl.handle.net/10344/4185
dc.description peer-reviewed en_US
dc.description.abstract Group IV Nanowires have strong potential for several biomedical applications. However, to date their use remains limited because many are synthesised using heavy metal seeds and functionalised using organic ligands to make the materials water dispersible. This can result in unpredicted toxic side effects for mammalian cells cultured on the wires. Here, we describe an approach to make seedless and ligand free Germanium nanowires water dispersible using glutamic acid, a natural occurring amino acid that alleviates the environmental and health hazards associated with traditional functionalisation materials. We analysed the treated material extensively using Transmission electron microscopy (TEM), High resolution-TEM, and scanning electron microscope (SEM). Using a series of state of the art biochemical and morphological assays, together with a series of complimentary and synergistic cellular and molecular approaches, we show that the water dispersible germanium nanowires are non-toxic and are biocompatible. We monitored the behaviour of the cells growing on the treated germanium nanowires using a real time impedance based platform (xCELLigence) which revealed that the treated germanium nanowires promote cell adhesion and cell proliferation which we believe is as a result of the presence of an etched surface giving rise to a collagen like structure and an oxide layer. Furthermore this study is the first to evaluate the associated effect of Germanium nanowires on mammalian cells. Our studies highlight the potential use of water dispersible Germanium Nanowires in biological platforms that encourage anchorage-dependent cell growth. en_US
dc.language.iso eng en_US
dc.publisher Public Library of Science en_US
dc.relation.ispartofseries PLOS One;9 (9), e108006
dc.relation.uri http://dx.doi.org/10.1371/journal.pone.0108006
dc.subject biomedical applications en_US
dc.subject nonowires en_US
dc.title Promoting cell proliferation using water dispersible germanium nanowires. en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.date.updated 2014-11-24T18:54:36Z
dc.description.version PUBLISHED
dc.identifier.doi 10.1371/journal.pone.0108006
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 1570959
dc.internal.rssid 1573595
dc.internal.copyrightchecked Yes
dc.description.status peer-reviewed


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