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Characterization of nanoporous gold electrodes for bioelectrochemical applications

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Show simple item record Scanlon, Micheál D. Salaj-Kośla, Urszula Belochapkine, Serguei MacAodha, Domhnall Leech, Dónal Ding, Yi Magner, Edmond 2014-10-30T10:13:04Z 2014-10-30T10:13:04Z 2012
dc.description peer-reviewed en_US
dc.description.abstract The high surface areas of nanostructured electrodes can provide for significantly enhanced surface loadings of electroactive materials. The fabrication and characterization of nanoporous gold (np-Au) substrates as electrodes for bioelectrochemical applications is described. Robust np-Au electrodes were prepared by sputtering a gold-silver alloy onto a glass support and subsequent dealloying of the silver component. Alloy layers were prepared with either a uniform or nonuniform distribution of silver and, post dealloying, showed clear differences in morphology on characterization with scanning electron microscopy. Redox reactions under kinetic control, in particular measurement of the charge required to strip a gold oxide layer, provided the most accurate measurements of the total electrochemically addressable electrode surface area, A(real). Values of A(real) up to 28 times that of the geometric electrode surface area, A(geo), were obtained. For diffusion-controlled reactions, overlapping diffusion zones between adjacent nanopores established limiting semi-infinite linear diffusion fields where the maximum current density was dependent on A(geo). The importance of measuring the surface area available for the immobilization was determined using the redox protein, cyt c. The area accessible to modification by a biological macromolecule, A(macro), such as cyt c was reduced by up to 40% compared to A(real), demonstrating that the confines of some nanopores were inaccessible to large macromolecules due to steric hindrances. Preliminary studies on the preparation of np-Au electrodes modified with osmium redox polymer hydrogels and Myrothecium verrucaria bilirubin oxidase (MvBOD) as a biocathode were performed; current densities of 500 mu A cm(-2) were obtained in unstirred solutions. en_US
dc.language.iso eng en_US
dc.publisher American Chemical Society en_US
dc.relation info:eu-repo/grantAgreement/EC/FP7/229255
dc.relation.ispartofseries Langmuir;28 (4), pp. 2251-2261
dc.rights This document is the unedited author's version of a Submitted Work that was subsequently accepted for publication in Langmuir, copyright © American Chemical Society after peer review. To access the final edited and published work, see en_US
dc.subject nanoporous gold en_US
dc.subject electrochemical characterization en_US
dc.subject cytochrome c en_US
dc.subject bilirubin oxidase en_US
dc.subject biosensor en_US
dc.subject biocathode en_US
dc.subject biofuel cell en_US
dc.title Characterization of nanoporous gold electrodes for bioelectrochemical applications en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US 2014-10-29T12:33:14Z
dc.description.version ACCEPTED
dc.contributor.sponsor ERC en_US
dc.contributor.sponsor INSPIRE en_US
dc.relation.projectid project 3D-NanoBioDevice NMP4-SL-2009-229255 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 1387591
dc.internal.copyrightchecked Yes
dc.description.status peer-reviewed

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