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Direct electron transfer of trametes hirsuta laccase adsorbed at unmodified nanoporous gold electrodes

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dc.contributor.author Salaj-Kośla, Urszula
dc.contributor.author Pöller, Sascha
dc.contributor.author Schuhmann, Wolfgang
dc.contributor.author Shleev, Sergey
dc.contributor.author Magner, Edmond
dc.date.accessioned 2014-10-28T16:38:30Z
dc.date.available 2014-10-28T16:38:30Z
dc.date.issued 2013
dc.identifier.citation Salaj-Kosla, U,Poller, S,Schuhmann, W,Shleev, S,Magner, E (2013) 'Direct electron transfer of Trametes hirsuta laccase adsorbed at unmodified nanoporous gold electrodes'. Bioelectrochemistry, 91 :15-20. en_US
dc.identifier.uri http://hdl.handle.net/10344/4112
dc.description peer-reviewed en_US
dc.description.abstract The enzyme Trametes hirsuta laccase undergoes direct electron transfer at unmodified nanoporous gold electrodes, displaying a current density of 28 mu A/cm(2). The response indicates that ThLc was immobilised at the surface of the nanopores in a manner which promoted direct electron transfer, in contrast to the absence of a response at unmodified polycrystalline gold electrodes. The bioelectrocatalytic activity of ThLc modified nanoporous gold electrodes was strongly dependent on the presence of halide ions. Fluoride completely inhibited the enzymatic response, whereas in the presence of 150 mM Cl-, the current was reduced to 50% of the response in the absence of Cl-. The current increased by 40% when the temperature was increased from 20 degrees C to 37 degrees C. The response is limited by enzymatic and/or enzyme electrode kinetics and is 30% of that observed for ThLc co-immobilised with an osmium redox polymer. (C) 2012 Elsevier B.V. All rights reserved. en_US
dc.language.iso eng en_US
dc.publisher Elsevier
dc.relation info:eu-repo/grantAgreement/EC/FP7/229255 en_US
dc.relation.ispartofseries Bioelectrochemistry;91, pp. 15-20
dc.relation.uri http://dx.doi.org/10.1016/j.bioelechem.2012.11.001
dc.rights This is the author’s version of a work that was accepted for publication in Bioelectrochemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Bioelectrochemistry, 91, pp. 15-20, http://dx.doi.org/10.1016/j.bioelechem.2012.11.001 en_US
dc.subject nanoporous gold electrodes en_US
dc.subject trametes hirsuta laccase en_US
dc.subject biocathode en_US
dc.subject bioelectrochemistry en_US
dc.title Direct electron transfer of trametes hirsuta laccase adsorbed at unmodified nanoporous gold electrodes en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.date.updated 2014-10-28T16:24:15Z
dc.description.version ACCEPTED
dc.contributor.sponsor ERC en_US
dc.contributor.sponsor HEA en_US
dc.contributor.sponsor INSPIRE en_US
dc.relation.projectid 3D-nanobiodevices (NMP4-SL-2009-229255) en_US
dc.relation.projectid PRTLI4 programme en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 1437009
dc.internal.copyrightchecked Yes
dc.description.status peer-reviewed


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