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Assessment of three epigenotypes in colorectal cancer by combined bisulphite restriction analysis.

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dc.contributor.author Karpinski, Pawel
dc.contributor.author Szmida, Elżbieta
dc.contributor.author Misiak, Blazej
dc.contributor.author Ramsey, David
dc.contributor.author Leszczynski, Przemyslaw
dc.contributor.author Bebenek, Marek
dc.contributor.author Sedziak, Tomasz
dc.contributor.author Grzebieniak, Zygmunt
dc.contributor.author Jonkisz, Anna
dc.contributor.author Lebioda, Arleta
dc.contributor.author Sasiadek, Maria Malgorzata
dc.date.accessioned 2014-07-22T10:19:11Z
dc.date.available 2014-07-22T10:19:11Z
dc.date.issued 2012
dc.identifier.uri http://hdl.handle.net/10344/3899
dc.description peer-reviewed en_US
dc.description.abstract Background: Recent investigations have demonstrated the clear heterogeneity of sporadic colorectal cancer (CRC) with regard to CpG island methylation. Two unsupervised cluster analyses revealed that CRCs form three distinct DNA methylation subsets, which are referred to as the high-, intermediate- and low-methylation epigenotypes (HME, IME, and LME, respectively). A recent study by Yagi et al. found a fairly sensitive and specific identification of HME, IME and LME using two marker panels analysed by MALDI-TOF mass spectrometry (MassARRAY). However, the expensive equipment required for this method substantially increases the cost and complexity of the assay. Findings: In this article, we demonstrate the assessment of HME, IME and LME in a group of 233 sporadic CRCs using seven markers proposed by Yagi et al. The DNA methylation of each marker was quantified using combined bisulphite restriction analysis (COBRA) together with an analysis of various genetic factors associated with CRC (the BRAF and KRAS mutations and microsatellite instability (MSI)). The baseline methylation of each marker was generated from pooled DNA isolated from 50 normal colon tissues. Conclusions: We demonstrate that the correlation of HME, IME and LME epigenotyped by COBRA using different molecular classifiers is similar to that achieved by MassARRAY. Therefore, epigenotyping CRCs using COBRA is a simple, specific and cost-effective method that has the potential to be widely used in CRC research. en_US
dc.language.iso eng en_US
dc.publisher Wiley en_US
dc.relation.ispartofseries Molecular Carcinogenesis;51, (12), pp. 1003-1008
dc.relation.uri http://dx.doi.org/10.1002/mc.20871
dc.rights This is the author's version of the following article:The definitive version is available at www.blackwell-synergy.com" en_US
dc.subject colorectal cancer en_US
dc.subject epigenotypes en_US
dc.subject methylation en_US
dc.subject methylator en_US
dc.title Assessment of three epigenotypes in colorectal cancer by combined bisulphite restriction analysis. en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.contributor.sponsor SFI en_US
dc.relation.projectid 07/MI/012 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


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