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Protein tyrosine phosphatase receptor-like genes are frequently hypermethylated in sporadic colorectal cancer

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dc.contributor.author Laczmanska, Izabela
dc.contributor.author Karpinski, Pawel
dc.contributor.author Bebenek, Marek
dc.contributor.author Sedziak, Tomasz
dc.contributor.author Ramsey, David
dc.contributor.author Szmida, Elżbieta
dc.contributor.author Sasiadek, Maria Malgorzata
dc.date.accessioned 2014-07-01T14:43:59Z
dc.date.available 2014-07-01T14:43:59Z
dc.date.issued 2013
dc.identifier.uri http://hdl.handle.net/10344/3874
dc.description peer-reviewed en_US
dc.description.abstract Introduction: The activity of phosphatases could be influenced by genetic, as well as epigenetic alterations. In our study we have investigated the methylation status of four PTPRs: PTPRM, PTPRT, PTPRR and PTPRZ1, which were pre-selected using microarray techniques as being alternatively methylated in sporadic colorectal cancer. Materials and methods: The analyses were carried out on 131 surgical specimens obtained from sporadic colorectal cancer patients. The methylation status of the four genes was examined using MSP. Results: The analysis of promoter methylation using an Illumina 27K microarray revealed four protein tyrosine phosphatases PTPRM, PTPRT, PTPRR and PTPRZ1 as being hypermethylated with beta-value ≥ 0.2 and p≤0.05. Subsequent analysis using MSP confirmed these observations - the frequency of promoter methylation was significantly higher in tumor cells compared to matched normal tissue for each of the analyzed genes. There was no association observed between the methylation status of PTPRs and either CIMP, K-ras (codon 12) and BRAF (exon 15, V600E) mutations or tumor localization (proximal/distal). The results of our study show a statistically significant difference between promoter methylation in cancerous and healthy tissue. This result supports the hypothesis that the PTPR family plays an important role in the etiology of colorectal cancer. en_US
dc.language.iso eng en_US
dc.publisher Nature Publishing Group en_US
dc.relation.ispartofseries Journal of Human Genetics;58 (1), pp. 11-5
dc.relation.uri http://dx.doi.org/ 10.1038/jhg.2012.119
dc.subject colorectal cancer en_US
dc.subject methylation en_US
dc.subject protein tyrosine phosphatase receptor-like en_US
dc.subject PTPRM en_US
dc.subject PTPRT en_US
dc.subject PTPRR en_US
dc.subject PTPRZ1 en_US
dc.title Protein tyrosine phosphatase receptor-like genes are frequently hypermethylated in sporadic colorectal cancer en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.contributor.sponsor State Committee for Scientific Research, Polish Ministry for Scientific Research and Information Technology en_US
dc.contributor.sponsor European Social Fund, Human Capital, National Cohesion Strategy en_US
dc.contributor.sponsor SFI en_US
dc.relation.projectid (grant no. N N401 601438), 2010-2013 en_US
dc.relation.projectid Contract No:UDA-POKL 04.01.01-00-010/08-00 en_US
dc.relation.projectid 07/MI012 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


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