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Microarray-based analysis of recombinant protein production in E.coli

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dc.contributor.author O'Dwyer, Ronan
dc.contributor.author Hu, Xuejun
dc.contributor.author Pelizzola, Mattia
dc.contributor.author Kolaj-Robin, Olga
dc.contributor.author Foti, Marti
dc.contributor.author Ricciardi-Castagnoli, Paola
dc.contributor.author Wall, J.G.
dc.date.accessioned 2014-04-24T10:45:57Z
dc.date.available 2014-04-24T10:45:57Z
dc.date.issued 2006
dc.identifier.uri http://hdl.handle.net/10344/3786
dc.description peer-reviewed en_US
dc.description.abstract The production of heterologous proteins in E. coli is a powerful tool in the generation of many important biotechnological and medical products. Despite its widespread use as an expression host, however, yields of correctly folded, functional protein are frequently low in E. coli. This is due largely to the formation of insoluble protein aggregates and to premature lysis of the bacterial cells. We, and others, have previously shown that the cell lysis phenomenon associated with recombinant protein production in E. coli is not a direct result of synthesis of heterologous proteins [1], [2]. Instead, protein production triggers a global stress response in the bacterium, but the mechanism by which cell lysis subsequently occurs remains unclear [3]. en_US
dc.language.iso eng en_US
dc.publisher BioMed Central en_US
dc.relation.ispartofseries Microbial Cell Factories;5 (Suppl 1): P4
dc.relation.uri http://dx.doi.org/doi:10.1186/1475-2859-5-S1-P4
dc.subject E.coli en_US
dc.subject biotechnological and medical products en_US
dc.title Microarray-based analysis of recombinant protein production in E.coli en_US
dc.type info:eu-repo/semantics/other en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


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