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The effect of environmental conditions on expression of Bacteroides fragilis and Bacteroides thetaiotaomicron C10 protease genes

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dc.contributor.author Thornton, Roibeard F.
dc.contributor.author Murphy, Elizabeth C.
dc.contributor.author Kagawa, Todd F.
dc.contributor.author O'Toole, Paul W.
dc.contributor.author Cooney, Jakki C.
dc.date.accessioned 2014-04-02T11:55:36Z
dc.date.available 2014-04-02T11:55:36Z
dc.date.issued 2012
dc.identifier.uri http://hdl.handle.net/10344/3754
dc.description peer-reviewed en_US
dc.description.abstract Background: Bacteroides fragilis and Bacteroides thetaiotaomicron are members of the normal human intestinal microbiota. However, both organisms are capable of causing opportunistic infections, during which the environmental conditions to which the bacteria are exposed change dramatically. To further explore their potential for contributing to infection, we have characterized the expression in B. thetaiotaomicron of four homologues of the gene encoding the C10 cysteine protease SpeB, a potent extracellular virulence factor produced by Streptococcus pyogenes. Results: We identified a paralogous set of genes (btp genes) in the B. thetaiotaomicron genome, that were related to C10 protease genes we recently identified in B. fragilis. Similar to C10 proteases found in B. fragilis, three of the B. thetaiotaomicron homologues were transcriptionally coupled to genes encoding small proteins that are similar in structural architecture to Staphostatins, protease inhibitors associated with Staphopains in Staphylococcus aureus. The expression of genes for these C10 proteases in both B. fragilis and B. thetaiotaomicron was found to be regulated by environmental stimuli, in particular by exposure to oxygen, which may be important for their contribution to the development of opportunistic infections. Conclusions: Genes encoding C10 proteases are increasingly identified in operons which also contain genes encoding proteins homologous to protease inhibitors. The Bacteroides C10 protease gene expression levels are responsive to different environmental stimuli suggesting they may have distinct roles in the bacterial-host interaction. en_US
dc.language.iso eng en_US
dc.publisher BioMed Central en_US
dc.relation.ispartofseries BMC Microbiology;12: 190
dc.relation.uri http://www.biomedcentral.com/1471-2180/12/190
dc.subject Bacteroides fragilis en_US
dc.subject DNA en_US
dc.title The effect of environmental conditions on expression of Bacteroides fragilis and Bacteroides thetaiotaomicron C10 protease genes en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.contributor.sponsor SFI en_US
dc.contributor.sponsor IRCSET en_US
dc.contributor.sponsor Department of Agriculture, Fisheries and Food en_US
dc.relation.projectid 08/RFP/BMT1596 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 1411349


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