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The Rab11 effector protein FIP1 regulates adiponectin trafficking and secretion

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Show simple item record Carson, Brian P. Del Bas, Josep Maria Moreno-Navarrete, Jose Maria Fernandez-Real, Jose Manuel Mora, Silvia 2013-10-08T15:00:59Z 2013-10-08T15:00:59Z 2013
dc.identifier.citation Carson BP, Del Bas JM, Moreno-Navarrete JM, Fernandez-Real JM, Mora S (2013) 'The Rab11 Effector Protein FIP1 Regulates Adiponectin Trafficking and Secretion'. Plos One, 8 (9). en_US
dc.description peer-reviewed en_US
dc.description.abstract Adiponectin is an adipokine secreted by white adipocytes involved in regulating insulin sensitivity in peripheral tissues. Secretion of adiponectin in adipocytes relies on the endosomal system, however, the intracellular machinery involved in mediating adiponectin release is unknown. We have previously reported that intracellular adiponectin partially compartmentalizes with rab 5 and rab11, markers for the early/sorting and recycling compartments respectively. Here we have examined the role of several rab11 downstream effector proteins (rab11 FIPs) in regulating adiponectin trafficking and secretion. Overexpression of wild type rab11 FIP1, FIP3 and FIP5 decreased the amount of secreted adiponectin expressed in HEK293 cells, whereas overexpression of rab11 FIP2 or FIP4 had no effect. Furthermore shRNA-mediated depletion of FIP1 enhanced adiponectin release whereas knock down of FIP5 decreased adiponectin secretion. Knock down of FIP3 had no effect. In 3T3L1 adipocytes, endogenous FIP1 co-distributed intracellularly with endogenous adiponectin and FIP1 depletion enhanced adiponectin release without altering insulin-mediated trafficking of the glucose transporter Glut4. While adiponectin receptors internalized with transferrin receptors, there were no differences in transferrin receptor recycling between wild type and FIP1 depleted adipocytes. Consistent with its inhibitory role, FIP1 expression was decreased during adipocyte differentiation, by treatment with thiazolidinediones, and with increased BMI in humans. In contrast, FIP1 expression increased upon exposure of adipocytes to TNFα. In all, our findings identify FIP1 as a novel protein involved in the regulation of adiponectin trafficking and release. en_US
dc.language.iso eng en_US
dc.publisher Public Library of Science en_US
dc.relation.ispartofseries PLOS One;
dc.relation.ispartofseries ;8(9), e74687
dc.subject adiponectin en_US
dc.subject adipose tissue en_US
dc.subject secretion en_US
dc.title The Rab11 effector protein FIP1 regulates adiponectin trafficking and secretion en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US 2013-10-07T14:29:59Z
dc.description.version PUBLISHED
dc.identifier.doi 10.1371/journal.pone.0074687
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 1444683
dc.internal.copyrightchecked No
dc.description.status peer-reviewed

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