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Genotypic and phenotypic diversity of Ralstonia pickettii and Ralstonia insidiosa isolates from clinical and environmental sources including high-purity water. Diversity in Ralstonia pickettii

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dc.contributor.author Ryan, Michael P.
dc.contributor.author Pembroke, Tony J.
dc.contributor.author Adley, Catherine C.
dc.date.accessioned 2013-07-12T09:15:39Z
dc.date.available 2013-07-12T09:15:39Z
dc.date.issued 2011
dc.identifier.uri http://hdl.handle.net/10344/3216
dc.description peer-reviewed en_US
dc.description.abstract Background: Ralstonia pickettii is a nosocomial infectious agent and a significant industrial contaminant. It has been found in many different environments including clinical situations, soil and industrial high purity water. This study compares the phenotypic and genotypic diversity of a selection of strains of Ralstonia collected from a variety of sources. Results: Ralstonia isolates (fifty-nine) from clinical, industrial and environmental origins were compared genotypically using i) Species-specific-PCR, ii) PCR and sequencing of the 16S-23S rRNA Interspatial region (ISR) iii) the fliC gene genes, iv) RAPD and BOX-PCR and v) phenotypically using biochemical testing. The species specific- PCR identified fifteen out of fifty-nine designated R. pickettii isolates as actually being the closely related species R. insidiosa. PCR-ribotyping of the 16S-23S rRNA ISR indicated few major differences between the isolates. Analysis of all isolates demonstrated different banding patterns for both the RAPD and BOX primers however these were found not to vary significantly. Conclusions: R. pickettii species isolated from wide geographic and environmental sources appear to be reasonably homogenous based on genotypic and phenotypic characteristics. R. insidiosa can at present only be distinguished from R. pickettii using species specific PCR. R. pickettii and R. insidiosa isolates do not differ significantly phenotypically or genotypically based on environmental or geographical origin. en_US
dc.language.iso eng en_US
dc.publisher BioMed Central en_US
dc.relation.ispartofseries BMC Microbiology;11:194
dc.relation.uri http://dx.doi.org/10.1186/1471-2180-11-194
dc.subject Ralstonia pickettii en_US
dc.subject random amplified polymorphic DNA en_US
dc.subject PCR en_US
dc.subject genotyping en_US
dc.subject high-purity water en_US
dc.subject field gel-electrophoresis
dc.subject polymerase-chain-reaction
dc.subject common-source outbreak
dc.subject pseudomonas-pickettii
dc.subject burkholderia-cepacia
dc.subject phylogenetic trees
dc.subject sequence alignment
dc.subject ultrapure water
dc.subject identification
dc.subject strains
dc.title Genotypic and phenotypic diversity of Ralstonia pickettii and Ralstonia insidiosa isolates from clinical and environmental sources including high-purity water. Diversity in Ralstonia pickettii en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.contributor.sponsor University of Limerick, CES Department en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 1148091


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