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Detection of viral DNA sequences in sporadic colorectal cancers in relation to CpG island methylation and methylator phenotype

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dc.contributor.author Karpinski, Pawel
dc.contributor.author Myszka, Aleksander
dc.contributor.author Ramsey, David
dc.contributor.author Kielan, Wojciech
dc.contributor.author Sasiadek, Maria Malgorzata
dc.date.accessioned 2013-05-20T11:22:48Z
dc.date.available 2013-05-20T11:22:48Z
dc.date.issued 2011
dc.identifier.uri http://hdl.handle.net/10344/3102
dc.description peer-reviewed en_US
dc.description.abstract There is evidence that insertion of viral DNA into a mammalian genome can lead to alterations of methylation patterns. The aim of the present study was to examine the presence of DNA sequences of five human DNA viruses (assessed by PCR): JC polyoma virus (JCV), human adenovirus (AdV), Epstein-Barr virus (EBV), Kaposi sarcoma-associated herpesvirus (KSHV/HHV8) and human papillomavirus (HPV) in a cohort of 186 sporadic colorectal cancers (CRCs) and related these data with the methylation status of six CIMP-specific genes (MLH1, CACNA1G, NEUROG1, IGF2, SOCS1, RUNX3) and seven cancer-related genes markers (p16, MINT1, MINT2, MINT31, EN1, SCTR and INHBB) assessed by methylationspecific PCR in 186 and 134 CRC cases, respectively. The AdV, KSHV and HPV were detected in 4 (2%), 2 (1%) and 0 CRC cases, respectively and thus were excluded from further analyses. Althought, 19% and 9% of the CRCs were positive for EBV and JCV respectively, no associations between virus presence and CpG island methylation were found after correction for multiple testing. Our results demonstrate that the presence of DNA sequences of JCV and EBV in CRC is unrelated to the methylation of the 13 cancer-related CpG islands and CIMP. en_US
dc.language.iso eng en_US
dc.publisher Springer-Verlag en_US
dc.relation.ispartofseries 2011;32(4), pp. 653-659
dc.relation.uri http://dx.doi.org/10.1007/s13277-011-0165-6
dc.rights The original publication is available at www.springerlink.com en_US
dc.subject CIMP en_US
dc.subject virus en_US
dc.subject JCV en_US
dc.subject EBV en_US
dc.subject CRC en_US
dc.subject colorectal en_US
dc.title Detection of viral DNA sequences in sporadic colorectal cancers in relation to CpG island methylation and methylator phenotype en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.contributor.sponsor SFI en_US
dc.relation.projectid 07/MI/012 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


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