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In vivo assessment of potential probiotic Lactobacillus salivarius strains: evaluation of their establishment, persistence, and localisation in the murine gastrointestinal tract

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Show simple item record Murphy, Lisa Dunne, Colum P. Kiely, Barry Shanahan, Fergus O'Sullivan, Gerald C. Collins, Kevin J. 2013-04-22T11:11:25Z 2013-04-22T11:11:25Z 1999
dc.description peer-reviewed en_US
dc.description.abstract The enteric flora comprise approximately 95% of the total number of cells in the human body. Numerous studies have investigated potentially beneficial members of this microbial community due to their ability to elicit immune responses while also protecting against microbial pathogens. We have previously reported on the isolation and identification, from surgically-resected segments of the human gastrointestinal tract (GIT), of potential probiotic lactic acid bacteria (LAB). These bacterial strains exhibit potentially beneficial probiotic traits in vitro such as bile tolerance in the absence of deconjugation; gastric acid resistance; and adherence to epithelial cell lines. The objective of this study was to administer two strains of the previously-isolated LAB to mice over a period of 7 or 14 days in order to assess their ability to establish themselves within specific regions of the GIT. Throughout this feeding period, and for 4 days following cessation of feeding, the numbers of total culturable lactobacilli and of the administered LAB present in faeces were monitored. Spontaneous rifampicin resistant derivatives (50 mg:ml) of Lactobacillus salivarius subsp. salivarius UCC1(LM5) and Lb. salivarius subsp. salivarius UCC118(LM2) were generated to facilitate enumeration of the strains in GIT and faecal samples. Each potential probiotic strain was individually administered to Balb:c mice at a daily concentration of approximately 4.0 109 CFU. After 1 day of feeding, strains UCC1(LM5) and UCC118(LM2) were recovered from murine faeces at Log10 6.95 (1.18) CFU:g and Log10 6.33 (0.37) CFU:g, respectively. Interestingly, UCC118(LM2), which was originally isolated from the ileal-caecal region of the human GIT, was found to have become established in the corresponding region of the murine GIT regardless of the length of the feeding period. UCC118(LM2) was also found to persist in faeces for a period of up to 3 days following cessation of feeding. Administration of UCC1(LM5) and UCC118(LM2) did not result in any significant changes in the levels of indigenous bacteria culturable from faeces. In conclusion, human isolate Lb. salivarius subsp. sali6arius UCC118(LM2) was found to effectively colonise, and survive transit through, the murine gastrointestinal tract. Lactobacillus salivarius subsp. sali6arius UCC118 has been deposited at The National Collections of Industrial and Marine Bacteria (NCIMB) and accorded the accession number NCIMB40829. en_US
dc.language.iso eng en_US
dc.publisher Co-Action Publishing en_US
dc.relation.ispartofseries Microbial Ecology in Health and Disease;11(3), pp. 149-157
dc.subject probiotics en_US
dc.subject Lactobacillus salivarius en_US
dc.subject in vivo en_US
dc.subject feeding trials en_US
dc.subject mouse en_US
dc.subject gastrointestinal tract en_US
dc.subject colonisation en_US
dc.subject transit en_US
dc.title In vivo assessment of potential probiotic Lactobacillus salivarius strains: evaluation of their establishment, persistence, and localisation in the murine gastrointestinal tract en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.contributor.sponsor BIO Research Ireland en_US
dc.contributor.sponsor Irish Department of Agriculture, Food and the Marine en_US
dc.contributor.sponsor European Commission en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 1121581

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