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Preparation and analysis of environmental DNA: optimisation of techniques for phylogenetic analysis of ATAD sludge

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dc.contributor.author Piterina, Anna Valentinova
dc.contributor.author Pembroke, Tony J.
dc.date.accessioned 2012-10-09T08:05:28Z
dc.date.available 2012-10-09T08:05:28Z
dc.date.issued 2010
dc.identifier.citation Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology (Ed) Antonio Mendez Vilas en_US
dc.identifier.issn ISBN (13): 978-84-614-6195-0
dc.identifier.uri http://hdl.handle.net/10344/2589
dc.description peer-reviewed en_US
dc.description.abstract Non-culture based analysis of microbial populations via phylogenetic analysis is becoming an increasingly important tool in the determination of microbial communities and community flux in a large number of environmental niches. The results obtained are however only as good as the preparative techniques used to isolate the original templates and the PCR processing techniques used to amplify these templates. In thermal niches such as autothermal thermophilic aerobic digestion systems (ATAD) there are many features that limit the optimal recovery of microbial diversity. Such features include lysis and release of products from the microbial population such as nucleases and proteases which effect both template recovery and polymerase functionality, inhibitory substances from the environmental source and even the nature of the primers used to amplify the resultant templates. In optimisation of the extraction and amplification of environmental DNA from an ATAD system we have identified a number of issues that affect the recovery of diversity which may have widespread applicability to the recovery of diversity by molecular techniques from other environmental niches. Methods of optimisation and analysis of diversity recovery will be discussed. en_US
dc.description.sponsorship HEA en_US
dc.language.iso eng en_US
dc.publisher Formatex en_US
dc.relation.ispartofseries Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology;2, pp. 1533-1538
dc.rights © Formatex 2010 en_US
dc.subject ATAD en_US
dc.subject moleculat diversity en_US
dc.subject molecular phylogenetics en_US
dc.subject environmental microbiology en_US
dc.title Preparation and analysis of environmental DNA: optimisation of techniques for phylogenetic analysis of ATAD sludge en_US
dc.type info:eu-repo/semantics/article
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.type.restriction none en
dc.rights.accessrights info:eu-repo/semantics/closedAccess en_US


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