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Modulating the pro-apoptotic activity of cytochrome c at a biomimetic electrified interface

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dc.contributor.author Gamero-Quijano, Alonso
dc.contributor.author Bhattacharya, Shayon
dc.contributor.author Cazade, Pierre-André
dc.contributor.author Molina-Osorio, Andrés
dc.contributor.author Beecher, Cillian
dc.contributor.author Djeghader, Ahmed
dc.contributor.author Soulimane, Tewfik
dc.contributor.author Dossot, Manuel
dc.contributor.author Thompson, Damien
dc.contributor.author Herzog, Grégoire
dc.contributor.author Scanlon, Micheál D.
dc.date.accessioned 2021-11-19T09:15:49Z
dc.date.available 2021-11-19T09:15:49Z
dc.date.issued 2021
dc.identifier.uri http://hdl.handle.net/10344/10803
dc.description peer-reviewed en_US
dc.description.abstract Programmed cell death via apoptosis is a natural defence against excessive cell division, crucial for fetal development to maintenance of homeostasis and elimination of precancerous and senescent cells. Here, we demonstrate an electrified liquid biointerface that replicates the molecular machinery of the inner mitochondrial membrane at the onset of apoptosis. By mimicking in vivo cytochrome c (Cyt c) interactions with cell membranes, our platform allows us to modulate the conformational plasticity of the protein by simply varying the electrochemical environment at an aqueous-organic interface. We observe interfacial electron transfer between an organic electron donor decamethylferrocene and O2, electrocatalyzed by Cyt c. This interfacial reaction requires partial Cyt c unfolding, mimicking Cyt c in vivo peroxidase activity. As proof of concept, we use our electrified liquid biointerface to identify drug molecules, such as bifonazole, that can potentially down-regulate Cyt c and protect against uncontrolled neuronal cell death in neurodegenerative disorders. en_US
dc.language.iso eng en_US
dc.publisher American Association for the Advancement of Science en_US
dc.relation 716792 en_US
dc.relation.ispartofseries Science Advances;7, eabg4119
dc.subject excessive cell en_US
dc.subject apoptosis en_US
dc.title Modulating the pro-apoptotic activity of cytochrome c at a biomimetic electrified interface en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1126/sciadv.abg4119
dc.contributor.sponsor SFI en_US
dc.contributor.sponsor European Union (EU) en_US
dc.contributor.sponsor IRC en_US
dc.contributor.sponsor French Programme Investissement d’Avenir en_US
dc.relation.projectid 13/SIRG/2137 en_US
dc.relation.projectid GOIPD/2018/252 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US
dc.internal.rssid 3025409


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