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Gene expression analysis of mouse embryonic stem cells following levitation in an ultrasound standing wave trap

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Show simple item record Bazou, Despina Kearney, Roisin Mansergh, Fiona C. Bourdon, Celine Farrar, Jane Wride, Michael A. 2021-11-18T11:33:31Z 2021-11-18T11:33:31Z 2011
dc.description peer-reviewed en_US
dc.description.abstract In the present paper, gene expression analysis of mouse embryonic stem (ES) cells levitated in a novel ultrasound standing wave trap (USWT) (Bazou et al. 2005a) at variable acoustic pressures (0.08–0.85 MPa) and times (5–60 min) was performed. Our results showed that levitation of ES cells at the highest employed acoustic pressure for 60 min does not modify gene expression and cells maintain their pluripotency. Embryoid bodies (EBs) also expressed the early and late neural differentiation markers, which were also unaffected by the acoustic field. Our results suggest that the ultrasound trap microenvironment is minimally invasive as the biologic consequences of ES cell replication and EB differentiation proceed without significantly affecting gene expression. The technique holds great promise in safe cell manipulation techniques for a variety of applications including tissue engineering and regenerative medicine. en_US
dc.language.iso eng en_US
dc.publisher Elsevier en_US
dc.relation.ispartofseries Ultrasound in Medicine & Biology;37 (2), pp. 321-330
dc.subject regenerative medicine en_US
dc.subject embryonic stem (ES) en_US
dc.title Gene expression analysis of mouse embryonic stem cells following levitation in an ultrasound standing wave trap en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1016/j.ultrasmedbio.2010.10.019
dc.contributor.sponsor European Union (EU) en_US
dc.contributor.sponsor HRB en_US
dc.relation.projectid MTKD-CT-2006-042519 – Nanofab) en_US
dc.relation.projectid H01242 en_US
dc.relation.projectid T01060 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US

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