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Mechanically stimulated osteocytes promote the proliferation and migration of breast cancer cells via a potential CXCL1/2 mechanism

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Show simple item record Dwivedi, Anushree Kiely, Patrick A. Hoey, David A. 2021-09-09T10:19:39Z 2021-09-09T10:19:39Z 2021
dc.description peer-reviewed en_US
dc.description.abstract Bone represents the most common site for breast cancer metastasis. Bone is a highly dynamic organ that is constantly adapting to its biophysical environment, orchestrated largely by the resident osteocyte network. Osteocytes subjected to physiologically relevant biophysical conditions may therefore represent a source of key factors mediating breast cancer cell metastasis to bone. Therefore, we investigated the potential proliferative and migratory capacity of soluble factors released by mechanically stimulated osteocytes on breast cancer cell behaviour. Interestingly the secretome of mechanically stimulated osteocytes enhanced both the proliferation and migration of cancer cells when compared to the secretome of statically cultured osteocytes, demonstrating that mechanical stimuli is an important physiological stimulus that should be considered when identifying potential targets. Using a cytokine array, we further identified a group of mechanically activated cytokines in the osteocyte secretome, which potentially drive breast cancer metastasis. In particular, CXCL1 and CXCL2 cytokines are highly expressed, mechanically regulated, and are known to interact with one another. Lastly, we demonstrate that these specific factors enhance breast cancer cell migration independently and in a synergistic manner, identifying potential osteocyte derived factors mediating breast cancer metastasis to bone. en_US
dc.language.iso eng en_US
dc.publisher Elsevier en_US
dc.relation 336882 en_US
dc.relation.ispartofseries Biochemical and Biophysical Research Communications;534, pp. 14-20
dc.rights This is the author’s version of a work that was accepted for publication in Biochemical and Biophysical Research Communications . Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochemical and Biophysical Research Communications, 2021, 534, pp. 14-20 en_US
dc.subject bone en_US
dc.subject breast cancer en_US
dc.subject CXCL en_US
dc.subject fluid shear en_US
dc.subject mechanobiology en_US
dc.title Mechanically stimulated osteocytes promote the proliferation and migration of breast cancer cells via a potential CXCL1/2 mechanism en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1016/j.bbrc.2020.12.016
dc.contributor.sponsor IRC en_US
dc.contributor.sponsor ERC en_US
dc.contributor.sponsor European Union (EU) en_US
dc.contributor.sponsor SFI en_US
dc.relation.projectid GOIPG/2014/1378/ en_US
dc.relation.projectid 336882 en_US
dc.relation.projectid 19/FFP/6533 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US

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