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On-chip glucose detection based on glucose oxidase immobilized on a platinum-modified, gold microband electrode

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Show simple item record Madden, Julia Barrett, Colm Laffir, Fathima R. Thompson, Michael Galvin, Paul O'Riordan, Alan 2021-08-05T10:21:57Z 2021-08-05T10:21:57Z 2021
dc.description peer-reviewed en_US
dc.description.abstract We report the microfabrication and characterization of gold microband electrodes on silicon using standard microfabrication methods, i.e., lithography and etching techniques. A two-step electrodeposition process was carried out using the on-chip platinum reference and gold counter electrodes, thus incorporating glucose oxidase onto a platinum-modified, gold microband electrode with an o-phenylenediamine and ß-cyclodextrin mixture. The as-fabricated electrodes were studied using optical microscopy, scanning electron microscopy, and atomic force microscopy. The two-step electrodeposition process was conducted in low sample volumes (50 µL) of both solutions required for biosensor construction. Cyclic voltammetry and electrochemical impedance spectroscopy were utilised for electrochemical characterization at each stage of the deposition process. The enzymatic based microband biosensor demonstrated a linear response to glucose from 2.5–15 mM, using both linear sweep voltammetry and chronoamperometric measurements in buffer-based solutions. The biosensor performance was examined in 30 µL volumes of fetal bovine serum. Whilst a reduction in the sensor sensitivity was evident within 100% serum samples (compared to buffer media), the sensor demonstrated linear glucose detection with increasing glucose concentrations (5–17 mM. en_US
dc.language.iso eng en_US
dc.publisher MDPI en_US
dc.relation.ispartofseries Biosensors;11, 249
dc.subject microband en_US
dc.subject glucose detection en_US
dc.subject enzymatic en_US
dc.subject electrochemical en_US
dc.subject microfabrication en_US
dc.subject silicon en_US
dc.title On-chip glucose detection based on glucose oxidase immobilized on a platinum-modified, gold microband electrode en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.3390/bios11080249
dc.contributor.sponsor SFI en_US
dc.contributor.sponsor Department of Agriculture, Food and the Marine en_US
dc.relation.projectid 12/RC/2289 en_US
dc.relation.projectid SFI/12/RC/2289-P2 en_US
dc.relation.projectid 16/RC/3835 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US

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