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Cell specific variation in viability in suspension in in vitro poiseuille flow conditions

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Show simple item record Connolly, Sinéad Á. Newport, David McGourty, Kieran Denis 2021-07-13T10:55:25Z 2021-07-13T10:55:25Z 2021
dc.description peer-reviewed en_US
dc.description.abstract The influence of Poiseuille flow on cell viability has applications in the areas of cancer metastasis, lab-on-a-chip devices and flow cytometry. Indeed, retaining cell viability is important in the emerging field of adoptive cell therapy, as cells need to be returned to patients’ bodies, while the viability of other cells, which are perhaps less accustomed to suspension in a fluidic environment, is important to retain in flow cytometers and other such devices. Despite this, it is unclear how Poiseuille flow affects cell viability. Following on from previous studies which investigated the viability and inertial positions of circulating breast cancer cells in identical flow conditions, this study investigated the influence that varying flow rate, and the corresponding Reynolds number has on the viability of a range of different circulating cells in laminar pipe flow including primary T cells, primary fibroblasts and neuroblastoma cells. It was found that Reynolds numbers as high as 9.13 had no effect on T-cells while the viabilities of neuroblastoma cells and intestinal fibroblasts were significantly reduced in comparison. This indicates that in vitro flow devices need to be tailored to cell-specific flow regimes en_US
dc.language.iso eng en_US
dc.publisher Nature en_US
dc.relation.ispartofseries Scientific Reports;11, 13997
dc.subject cell-specific en_US
dc.subject Poiseuille en_US
dc.title Cell specific variation in viability in suspension in in vitro poiseuille flow conditions en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1038/s41598-021-91865-1
dc.contributor.sponsor IRC en_US
dc.relation.projectid GOIPG/2016/1265 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US

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