University of Limerick Institutional Repository

Cell specific variation in viability in suspension in in vitro poiseuille flow conditions

DSpace Repository

You will not be able to submit new items to the ULIR while we upgrade to the new research repository. If you wish to add items, or have any questions about the new system, please contact the ULIR administrator at ir@ul.ie. We are sorry for any inconvenience.

Show simple item record

dc.contributor.author Connolly, Sinéad Á.
dc.contributor.author Newport, David
dc.contributor.author McGourty, Kieran Denis
dc.date.accessioned 2021-07-13T10:55:25Z
dc.date.available 2021-07-13T10:55:25Z
dc.date.issued 2021
dc.identifier.uri http://hdl.handle.net/10344/10340
dc.description peer-reviewed en_US
dc.description.abstract The influence of Poiseuille flow on cell viability has applications in the areas of cancer metastasis, lab-on-a-chip devices and flow cytometry. Indeed, retaining cell viability is important in the emerging field of adoptive cell therapy, as cells need to be returned to patients’ bodies, while the viability of other cells, which are perhaps less accustomed to suspension in a fluidic environment, is important to retain in flow cytometers and other such devices. Despite this, it is unclear how Poiseuille flow affects cell viability. Following on from previous studies which investigated the viability and inertial positions of circulating breast cancer cells in identical flow conditions, this study investigated the influence that varying flow rate, and the corresponding Reynolds number has on the viability of a range of different circulating cells in laminar pipe flow including primary T cells, primary fibroblasts and neuroblastoma cells. It was found that Reynolds numbers as high as 9.13 had no effect on T-cells while the viabilities of neuroblastoma cells and intestinal fibroblasts were significantly reduced in comparison. This indicates that in vitro flow devices need to be tailored to cell-specific flow regimes en_US
dc.language.iso eng en_US
dc.publisher Nature en_US
dc.relation.ispartofseries Scientific Reports;11, 13997
dc.subject cell-specific en_US
dc.subject Poiseuille en_US
dc.title Cell specific variation in viability in suspension in in vitro poiseuille flow conditions en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1038/s41598-021-91865-1
dc.contributor.sponsor IRC en_US
dc.relation.projectid GOIPG/2016/1265 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


Files in this item

This item appears in the following Collection(s)

Show simple item record

Search ULIR


Browse

My Account

Statistics