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Macroalgal protein hydrolysates from palmaria palmata influence the ‘incretin effect’ in vitro via DPP‑4 inhibition and upregulation of insulin, GLP‑1 and GIP secretion

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Show simple item record McLaughlin, C. M. Harnedy-Rothwell, Pádraigín A. Laferty, R. A. Sharkey, S. Parthsarathy, V. Allsopp, Philip J. McSorley, E. M. FitzGerald, Richard J. O'Harte, F. P. M. 2021-06-28T10:52:09Z 2021-06-28T10:52:09Z 2021
dc.description peer-reviewed en_US
dc.description.abstract Purpose This study investigated metabolic benefits of protein hydrolysates from the macroalgae Palmaria palmata, previously shown to inhibit dipeptidylpeptidase-4 (DPP-4) activity in vitro. Methods Previously, Alcalase/Flavourzyme-produced P. palmata protein hydrolysate (PPPH) improved glycaemia and insulin production in streptozotocin-induced diabetic mice. Here the PPPH, was compared to alternative Alcalase, bromelain and Promod-derived hydrolysates and an unhydrolysed control. All PPPH’s underwent simulated gastrointestinal digestion (SGID) to establish oral bioavailability. PPPH’s and their SGID counterparts were tested in pancreatic, clonal BRIN-BD11 cells to assess their insulinotropic efect and associated intracellular mechanisms. PPPH actions on the incretin effect were assessed via measurement of DPP-4 activity, coupled with GLP-1 and GIP release from GLUTag and STC-1 cells, respec tively. Acute in vivo effects of Alcalase/Flavourzyme PPPH administration on glucose tolerance and satiety were assessed in overnight-fasted mice. Results PPPH’s (0.02–2.5 mg/ml) elicited varying insulinotropic efects (p<0.05–0.001). SGID of the unhydrolysed protein control, bromelain and Promod PPPH’s retained, or improved, bioactivity regarding insulin secretion, DPP-4 inhibition and GIP release. Insulinotropic effects were retained for all SGID-hydrolysates at higher PPPH concentrations. DPP-4 inhibitory effects were confrmed for all PPPH’s and SGID counterparts (p<0.05–0.001). PPPH’s were shown to directly influence the incretin effect via upregulated GLP-1 and GIP (p<0.01–0.001) secretion in vitro, largely retained after SGID. Alcalase/Flavourzyme PPPH produced the greatest elevation in cAMP (p<0.001, 1.7-fold), which was fully retained post-SGID. This hydrolysate elicited elevations in intracellular calcium (p<0.01) and membrane potential (p<0.001). In acute in vivo settings, Alcalase/Flavourzyme PPPH improved glucose tolerance (p<0.01–0.001) and satiety (p<0.05–0.001). Conclusion Bioavailable PPPH peptides may be useful for the management of T2DM and obesity. en_US
dc.language.iso eng en_US
dc.publisher Springer en_US
dc.relation.ispartofseries European Journal of Nutrition;
dc.subject Antidiabetic en_US
dc.subject Dipeptidylpeptidase-4 en_US
dc.subject Dulse en_US
dc.title Macroalgal protein hydrolysates from palmaria palmata influence the ‘incretin effect’ in vitro via DPP‑4 inhibition and upregulation of insulin, GLP‑1 and GIP secretion en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1007/s00394-021-02583-3
dc.contributor.sponsor Department of Agriculture, Food and the Marine en_US
dc.contributor.sponsor Department of Education & Learning Northern Ireland en_US
dc.relation.projectid 11/F/063 en_US
dc.relation.projectid 11/F064 en_US
dc.relation.projectid 13/F536 en_US
dc.relation.projectid 14/F873 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US

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