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Detection of Pseudomonas aeruginosa quorum sensing molecules at an electrified liquid|liquid micro-interface through facilitated proton transfer

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dc.contributor.author Burgoyne, Edward D.
dc.contributor.author Molina-Osorio, Andrés
dc.contributor.author Moshrefi, Reza
dc.contributor.author Shanahan, Rachel
dc.contributor.author McGlacken, Gerard P.
dc.contributor.author Stockmann, Talia Jane
dc.contributor.author Scanlon, Micheál D.
dc.date.accessioned 2021-06-04T10:36:21Z
dc.date.issued 2020
dc.identifier.citation Burgoyne, Edward D.; Molina-Osorio, Andrés F.; Moshrefi, Reza; Shanahan, Rachel; McGlacken, Gerard P.; Stockmann, Talia Jane and Scanlon, Micheál D. (2020) 'Detection of Pseudomonas aeruginosa quorum sensing molecules at an electrified liquid|liquid micro-interface through facilitated proton transfer'. The Analyst, 145 (21):7000-7008. en_US
dc.identifier.issn 1364-5528
dc.identifier.uri http://hdl.handle.net/10344/10141
dc.description peer-reviewed en_US
dc.description The full text of this article will not be available in ULIR until the embargo expires on the 01/09/2021
dc.description.abstract Miniaturization of electrochemical detection methods for point-of-care-devices is ideal for their integration and use within healthcare environments. Simultaneously, the prolific pathogenic bacteria Pseudomonas aeruginosa poses a serious health risk to patients with compromised immune systems. Recognizing these two factors, a proof-of-concept electrochemical method employing a micro-interface between water and oil (w/o) held at the tip of a pulled borosilicate glass capillary is presented. This method targets small molecules produced by P. aeruginosa colonies as signalling factors that control colony growth in a pseudo-multicellular process known as quorum sensing (QS). The QS molecules of interest are 4-hydroxy-2-heptylquinoline (HHQ) and 2-heptyl-3,4-dihydroxyquinoline (PQS, Pseudomonas quinolone signal). Hydrophobic HHQ and PQS molecules, dissolved in the oil phase, were observed electrochemically to facilitate proton transfer across the w/o interface. This interfacial complexation can be exploited as a facile electrochemical detection method for P. aeruginosa and is advantageous as it does not depend on the redox activity of HHQ/PQS. Interestingly, the limit-of-linearity is reached as [H+] ≈ [ligand]. Density functional theory calculations were performed to determine the proton affinities and gas-phase basicities of HHQ/PQS, as well as elucidate the likely site of stepwise protonation within each molecule. en_US
dc.language.iso eng en_US
dc.publisher Royal Society of Chemistry en_US
dc.relation 716792 en_US
dc.relation.ispartofseries Analyst;145, pp. 7000-7008
dc.relation.uri https://pubs.rsc.org/lv/content/articlelanding/2020/an/d0an01245a/unauth#!divAbstract
dc.relation.uri https://doi.org/10.1039/D0AN01245A
dc.rights © 2020 Royal Society of Chemistry. Personal use of this material is permitted. Permission from Royal Society of Chemistry must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works en_US
dc.subject molecules en_US
dc.subject Pseudomonas aeruginosa en_US
dc.title Detection of Pseudomonas aeruginosa quorum sensing molecules at an electrified liquid|liquid micro-interface through facilitated proton transfer en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.date.updated 2021-06-02T14:28:30Z
dc.description.version ACCEPTED
dc.identifier.doi 10.1039/d0an01245a
dc.contributor.sponsor SFI en_US
dc.contributor.sponsor ERC en_US
dc.contributor.sponsor European Union (EU) en_US
dc.contributor.sponsor IRC en_US
dc.relation.projectid GOIPG/2016/1217 en_US
dc.relation.projectid 716792 en_US
dc.relation.projectid 13/SIRG/2137 en_US
dc.date.embargoEndDate 2021-09-01
dc.embargo.terms 2021-09-01 en_US
dc.rights.accessrights info:eu-repo/semantics/embargoedAccess en_US
dc.internal.rssid 3006685
dc.internal.copyrightchecked Yes
dc.identifier.journaltitle The Analyst
dc.description.status peer-reviewed


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