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Development of a multiplex assay to determine the expression of mitochondrial genes in human skeletal muscle

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dc.contributor.author Aird, Thomas.P.
dc.contributor.author Farquharson, Andrew J.
dc.contributor.author Drew, Janice E.
dc.contributor.author Carson, Brian P.
dc.date.accessioned 2021-05-26T07:22:38Z
dc.date.available 2021-05-26T07:22:38Z
dc.date.issued 2021
dc.identifier.uri http://hdl.handle.net/10344/10110
dc.description peer-reviewed en_US
dc.description.abstract Skeletal muscle is an important endocrine tissue demonstrating plasticity in response to external stimuli, including exercise and nutrition. Mitochondrial biogenesis is a common hallmark of adaptations to aerobic exercise training. Furthermore, altered expression of several genes implicated in the regulation of mitochondrial biogenesis, substrate oxidation and nicotinamide adenine dinucleotide (NAD+) biosynthesis following acute exercise underpins longer-term muscle metabolic adaptations. Gene expression is typically measured using real-time quantitative PCR platforms. However, interest has developed in the design of multiplex gene expression assays (GeXP) using the GenomeLab GeXP™ genetic analysis system, which can simultaneously quantify gene expression of multiple targets, holding distinct advantages in terms of throughput, limiting technical error, cost effectiveness, and quantifying gene coexpression. This study describes the development of a custom-designed GeXP assay incorporating the measurement of proposed regulators of mitochondrial biogenesis, substrate oxidation, and NAD+ biosynthetic capacity in human skeletal muscle and characterises the resting gene expression (overnight fasted and non-exercised) signature within a group of young, healthy, recreationally active males. The design of GeXP-based assays provides the capacity to more accurately characterise the regulation of a targeted group of genes with specific regulatory functions, a potentially advantageous development for future investigations of the regulation of muscle metabolism by exercise and/or nutrition. en_US
dc.language.iso eng en_US
dc.publisher Wiley en_US
dc.relation.ispartofseries Experimental Physiology;pp. 1–12
dc.subject gene expression en_US
dc.subject mitochondria en_US
dc.title Development of a multiplex assay to determine the expression of mitochondrial genes in human skeletal muscle en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1113/EP089557
dc.contributor.sponsor Carbery Food Ingredients en_US
dc.contributor.sponsor Scottish Government’s Rural and Environment Science and Analytical Services Division en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


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