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Environmental DNA monitoring of oncogenic viral shedding and genomic profiling of sea turtle fibropapillomatosis reveals unusual viral dynamics

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Show simple item record Farrell, Jessica A. Yetsko, Kelsey Whitmore, Liam Whilde, Jenny Eastman, Catherine B. Ramia, Devon Rollinson Thomas, Rachel Linser, Paul Creer, Simon Burkhalter, Brooke Schnitzler, Christine Duffy, David J. 2021-05-21T11:06:07Z 2021-05-21T11:06:07Z 2021
dc.description peer-reviewed en_US
dc.description.abstract Pathogen-induced cancers account for 15% of human tumors and are a growing concern for endangered wildlife. Fibropapillomatosis is an expanding virally and environmentally coinduced sea turtle tumor epizootic. Chelonid herpesvirus 5 (ChHV5) is implicated as a causative virus, but its transmission method and specific role in oncogenesis and progression is unclear. We applied environmental (e)DNA-based viral monitoring to assess viral shedding as a direct means of transmission, and the relationship between tumor burden, surgical resection and ChHV5 shedding. To elucidate the abundance and transcriptional status of ChHV5 across early, established, regrowth and internal tumors we conducted genomics and transcriptomics. We determined that ChHV5 is shed into the water column, representing a likely transmission route, and revealed novel temporal shedding dynamics and tumor burden correlations. ChHV5 was more abundant in the water column than in marine leeches. We also revealed that ChHV5 is latent in fibropapillomatosis, including early stage, regrowth and internal tumors; higher viral transcription is not indicative of poor patient outcome, and high ChHV5 loads predominantly arise from latent virus. These results expand our knowledge of the cellular and shedding dynamics of ChHV5 and can provide insights into temporal transmission dynamics and viral oncogenesis not readily investigable in tumors of terrestrial species. en_US
dc.language.iso eng en_US
dc.publisher Nature en_US
dc.relation 17-033R en_US
dc.relation.ispartofseries Communications Biology;4, 565
dc.subject Pathogen-induced cancers en_US
dc.subject Fibropapillomatosis en_US
dc.title Environmental DNA monitoring of oncogenic viral shedding and genomic profiling of sea turtle fibropapillomatosis reveals unusual viral dynamics en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.1038/s42003-021-02085-2
dc.contributor.sponsor The Sea Turtle Conservancy en_US
dc.contributor.sponsor Florida Sea Turtle en_US
dc.contributor.sponsor Sêr Cymru II en_US
dc.contributor.sponsor Gumbo Limbo en_US
dc.contributor.sponsor Horizon 2020 en_US
dc.contributor.sponsor IRC en_US
dc.relation.projectid 663830-BU115 en_US
dc.relation.projectid GOIPG/2020/1056 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US

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