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Dark field and coherent anti-stokes raman (DF-CARS) imaging of cell uptake of core-shell, magnetic-plasmonic nanoparticles

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dc.contributor.author Brennan, Grace
dc.contributor.author Ryan, Sally
dc.contributor.author Soulimane, Tewfik
dc.contributor.author Tofail, Syed A.M.
dc.contributor.author Silien, Christophe
dc.date.accessioned 2021-04-16T08:46:17Z
dc.date.available 2021-04-16T08:46:17Z
dc.date.issued 2021
dc.identifier.uri http://hdl.handle.net/10344/10002
dc.description peer-reviewed en_US
dc.description.abstract Magnetic-plasmonic, Fe3O4 -Au, core-shell nanoparticles are popular in many applications, most notably in therapeutics and diagnostics, and thus, the imaging of these nanostructures in biological samples is of high importance. These nanostructures are typically imaged in biological material by dark field scatter imaging, which requires an even distribution of nanostructures in the sample and, therefore, high nanoparticle doses, potentially leading to toxicology issues. Herein, we explore the nonlinear optical properties of magnetic nanoparticles coated with various thicknesses of gold using the open aperture z-scan technique to determine the nonlinear optical properties and moreover, predict the efficacy of the nanostructures in nonlinear imaging. We find that the magnetic nanoparticles coated with gold nano seeds and thinner gold shells (ca. 4 nm) show the largest nonlinear absorption coefficient β and imaginary part of the third-order susceptibility Im χ(3), suggesting that these nanostructures would be suitable contrast agents. Next, we combine laser dark field microscopy and epi-detected coherent anti-Stokes Raman (CARS) microscopy to image the uptake of magnetic-plasmonic nanoparticles in human pancreatic cancer cells. We show the epi-detected CARS technique is suitable for imaging of the magnetic-plasmonic nanoparticles without requiring a dense distribution of nanoparticles. This technique achieves superior nanoparticle contrasting over both epi-detected backscatter imaging and transmission dark field imaging, while also attaining label-free chemical contrasting of the cell. Lastly, we show the high biocompatibility of the Fe3O4 nanoparticles with ca. 4-nm thick Au shell at concentrations of 10–100 µg/ en_US
dc.language.iso eng en_US
dc.publisher MDPI en_US
dc.relation.ispartofseries Nanomaterials;11, (685)
dc.subject magnetic-plasmonic nanoparticles en_US
dc.subject coherent anti-Stokes Raman en_US
dc.subject contrast agents en_US
dc.subject nonlinear optics en_US
dc.title Dark field and coherent anti-stokes raman (DF-CARS) imaging of cell uptake of core-shell, magnetic-plasmonic nanoparticles en_US
dc.type info:eu-repo/semantics/article en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.identifier.doi 10.3390/nano11030685
dc.contributor.sponsor SFI en_US
dc.contributor.sponsor European Union (EU) en_US
dc.relation.projectid 13CDA2221 en_US
dc.relation.projectid 13/RC/2073 en_US
dc.rights.accessrights info:eu-repo/semantics/openAccess en_US


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