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Cell-seeded decellularised extracellular matrices as an advanced approach for tissue engineering

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dc.contributor.author Piterina, Anna Valentinova
dc.contributor.author Davis, Laura M.
dc.contributor.author Meaney, Claire L.
dc.contributor.author Cloonan, Aidan J.
dc.contributor.author Walsh, Michael T.
dc.contributor.author McGloughlin, Timothy M.
dc.date.accessioned 2011-09-29T15:13:46Z
dc.date.available 2011-09-29T15:13:46Z
dc.date.issued 2009
dc.identifier.uri http://hdl.handle.net/10344/1267
dc.description peer-reviewed en_US
dc.description.abstract Development and optimisation of therapeutic applications using biological scaffolds composed of naturally occurring extracellular matrix (ECM) have received significant attention in the field of tissue engineering. Histological and molecular investigations combined with comprehensive electron microscopy have shown that decellularised matrices from animal sources may vary in structure and biochemical properties. The source (bladder or small intestine submucosa), species, age of animal, and type of preparation of these matrices may contribute to the unique biophysical properties of the delivered product. Comparison of the thickness, topography, porosity and biomechanical properties of these matrices are presented. Vascular cell adhesion, migration and proliferation, and cell ingrowths on scaffold matrices were analysed using fluorescent labelling and laser confocal microscopy. ECM is shown to be a promising material for vascular tissue engineering and supports the growth, proliferation, anchoring and migration of human vasculature derived cells. Scaffold features tightly control the rate of adhesion and initiation of the proliferation. UBM scaffold containing an intact basal membrane may strongly influence the phenotype of the smooth muscle cells and control their proliferation-differentiation mechanisms. Data obtained will help realise and guide the research community with regard to the appropriate use of extracellular matrices derived from various animal organs based on their properties and suggests the possibility of conditioning this matrixes in order to influence their tissue and cell specificity before utilisation in clinical use. en_US
dc.language.iso eng en_US
dc.relation.ispartofseries ECCOMAS – International Conference On Tissue Engineering, Leiria, Portugal, July 9-11, 2009;
dc.subject decellularised extracellular matrices en_US
dc.subject tissue engineering en_US
dc.subject bioreactors en_US
dc.subject vascular endothelial cell en_US
dc.subject vascular smooth muscle cell en_US
dc.title Cell-seeded decellularised extracellular matrices as an advanced approach for tissue engineering en_US
dc.type Conference item en_US
dc.type.supercollection all_ul_research en_US
dc.type.supercollection ul_published_reviewed en_US
dc.type.restriction none en
dc.internal.authorcontactother Anna.Piterina@ul.ie


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